Detection and Quantification of Apple Stem Grooving Virus in Micropropagated Apple Plantlets Using Reverse-Transcription Droplet Digital PCR

Authors

  • Sung-Woong Kim, Hyo-Jeong Lee, Kang Hee Cho, Rae-Dong Jeong

Keywords:

apple stem grooving virus, detection, quantification, reverse transcription droplet digital polymerase chain reaction

Abstract

Apple stem grooving virus (ASGV) is a destructive viral pathogen of pome fruit trees that causes significant losses to fruit production worldwide. Obtaining ASGV-free propagation materials is essential to reduce economic losses, and accurate and sensitive detection methods to screen ASGV-free plantlets during in vitro propagation are urgently necessary. In this study, ASGV was sensitively and accurately quantified from in vitro propagated apple plantlets using a reverse transcription droplet digital polymerase chain reaction (RT-ddPCR) assay. The optimized RT-ddPCR assay was specific to other apple viruses, and was at least 10-times more sensitive than RT-real-time quantitative PCR assay. Furthermore, the optimized RT-ddPCR assay was validated for the detection and quantification of ASGV using micropropagated apple plantlet samples. This RT-ddPCR assay can be utilized for the accurate quantitative detection of ASGV infection in ASGV-free certification programs, and can thus contribute to the production of ASGV-free apple trees.

 

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Published

2022-08-28

How to Cite

Sung-Woong Kim, Hyo-Jeong Lee, Kang Hee Cho, Rae-Dong Jeong. (2022). Detection and Quantification of Apple Stem Grooving Virus in Micropropagated Apple Plantlets Using Reverse-Transcription Droplet Digital PCR. The Plant Pathology Journal, 38(4), 417–422. Retrieved from https://ppj-online.org/index.php/PPJ/article/view/1096

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Section

Articles